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Research Article

Cancer Research Frontiers. 2016 Feb; 2(1): 43-54. doi: 10.17980/2016.43

Camphorataimide B, a maleimide in mycelium of Antrodia camphorate, inhibits progression of human MDA-MB-231 breast cancer cells

 

Yean-Jang Lee1, Chi-Chou Huang2,3, Wea-Lung Lin2,4,, Chia-Hung Hung5, Pei-Yun Huang5, Tsui-Hwa Tseng6,7, *

  1. Department of Chemistry, National Changhua University of Education, No. 1, Jin-De Road, Changhua 500, Taiwan.
  2. School of Medicine, Chung Shan Medical University, 110, Sec.1, Jianguo N.Rd.,Taichung City 402,Taiwan.
  3. Division of Colorectal surgery Department of Surgery Chung Shan Medical University Hospital, 110, Sec.1, Jianguo N. Rd.,Taichung City 402,Taiwan.
  4. Department of Pathology, Chung Shan Medical University Hospital, 110, Sec.1, Jianguo N.Rd.,Taichung City 402,Taiwan.
  5. Institute of Biochemistry and Biotechnology, college of medicine, Chung Shan Medical University, 110, Sec.1, Jianguo N.Rd.,Taichung City 402,Taiwan.
  6. School of Medical Applied Chemistry, Chung Shan Medical University, No. 110, Section 1, 110,Sec.1,Jianguo N.Rd.,Taichung City 402,Taiwan.
  7. Department of Medical Education, Chung Shan Medical University Hospital, 110, Sec.1, Jianguo N.Rd.,Taichung City 402,Taiwan.
    † contribution as the first author

 

*Corresponding author: Tsui-Hwa Tseng, School of Medical Applied Chemistry, Chung Shan Medical University. No.110,Sec.1,Jianguo N.Rd.,Taichung City 40201,Taiwan. Phone: +886 4 24730022; Fax: +886 4 23248189. E-mail: tht@csmu.edu.tw

Citation: Yean-Jang Lee, et al. Camphorataimide B, a maleimide in mycelium of Antrodia camphorate, inhibits progression of human MDA-MB-231 breast cancer cells. Cancer Research Frontiers. 2016 Feb; 2(1): 43-54. doi: 10.17980/2016.43

Copyright: @ 2016 Yean-Jang Lee, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Competing Interests: The authors declare that there are no competing interests.

Received Oct 28, 2015; Revised Jan 7, 2016; Accepted Jan 9, 2016. Published Jan 22, 2016

 

ABSTRACT

Breast cancer is one of the most common malignancies among women worldwide. The degree of metastasis negatively affects a breast cancer patient’s prognosis and treatment benefits. In a previous investigation, camphorataimide B (Cam B), a maleimide derivative, was isolated from the mycelium of Antrodia camphorate, and inhibited cell cycle progression and tumor growth in MDA-MB-231 human breast cancer cells. However, it is not clear whether this compound exerts other anticancer effects. The results demonstrated that synthetic Cam B can inhibit the anchorage-independent growth of MDA-MB-231 breast cancer cells. In addition, Cam B decreased motility and invasion of MDA-MB-231 breast cancer cells. Moreover, Cam B reduced the expression of hypoxia-inducible factor-1α (HIF-1α) and its target gene product such as vimentin, cathepsin D and matrix metalloproteinase-2 (MMP-2), which play important roles in tumor progression. Additionally, Cam B reduced the phosphorylation of AKT and p65 NFκB, which associated the downregulation of HIF-1α. Furthermore, Cam B inhibited pulmonary colonization of MDA-MB-231 breast cancer cells in nude mice. By histological and gross examination of mouse lung, it showed that pretreated Cam B reduced the lung colonization of MDA-MB-231 breast cancer cells. The immunohistochemical data exhibited that pretreated Cam B decreased HIF-1α in lung section. These results demonstrate that Cam B reveals a novel role in inhibiting tumor progression.

Keywords: AKT, camphorataimide B, HIF-1α, p65 NF-κB.

 

 

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