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Case Report

Cancer Research Frontiers. 2016 Sept; 2(3): 416-426. doi: 10.17980/2016.416

Unusual thermal transition in the serum calorimetric profile of a patient diagnosed with multiple myeloma with secretion of monoclonal κ free light chains: a case report

Svetla Todinova1, Sashka Krumova1, Tonya Andreeva1, Keranka Dimitrova2, Lidia Gartcheva2, Stefka Germanova Taneva1,*

1Institute of Biophysics and Biomedical Engineering, Bulgarian Academy of Sciences, Sofia 1113, Bulgaria

2National Specialized Hospital for Active Treating of Haematological Diseases, Sofia 1756, Bulgaria

*Corresponding author: Stefka Germanova Taneva, DSc, Professor of Biophysics, Department of Biomacromolecules and Biomolecular Interactions, Institute of Biophysics and Biomedical Engineering, Bulgarian Academy of Sciences, Acad. G. Bonchev Str., Bl. 21, Sofia 1113, Bulgaria, Tel: 00359-29-79-26-25; Fax: 0035-98-72-37-87; Email: sgtaneva@gmail.com

Citation: Svetla Todinova, et al. Unusual thermal transition in the serum calorimetric profile of a patient diagnosed with multiple myeloma with secretion of monoclonal κ free light chains: a case report. Cancer Research Frontiers. 2016 Sept; 2(3): 416-426. doi: 10.17980/2016.416

Copyright: @ 2016 Svetla Todinova, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Competing Interests: The authors declare no competing financial interests.

Received Sept 28, 2016; Revised Dec 6, 2016; Accepted Dec 15, 2016. Published Dec 29, 2016

 

Abstract

Differential scanning calorimetry (DSC) gains speed and success in the last decade in the characterization of blood plasma and serum. Numerous publications reveal the potential of this technique to identify calorimetric markers specific for variety of diseases and their staging. In our previous works we have clearly demonstrated that DSC can serve to classify multiple myeloma cases in a number of calorimetric groups whose thermodynamic parameters are strongly affected by the level and isotype of the secreted monoclonal immunoglobulins or free light chains (FLC). In this report we present a case of multiple myeloma with secretion of monoclonal κ FLC (stage III according to ISS classification). High FLC level (about 20% from the total protein content) was found in the patient’s serum that remained persistent for the monitoring period of 1 year. The calorimetric profile of the serum revealed the occurrence of an unusual calorimetric transition at 46-47 °C, unique among nearly 500 multiple myeloma patients studied by us so far. This transition was assigned to unstable monoclonal free light chains that also led to the formation of amorphous aggregates (imaged by atomic force microscopy) in the patient’s serum. Additional studies of patients with similar calorimetric features are needed in order to relate the emergence of the 47 °C transition and protein aggregation to the disease activity status of multiple myeloma or to other pathology.

Key words: multiple myeloma, monoclonal free light chains, differential scanning calorimetry

 

 

 

 

 

 

 

 

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