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Review

Cancer Research Frontiers. 2015 Apr; 1(2): 119-126. doi: 10.17980/2015.119

Iterative data multiplexing (IDM) supports elucidation of drug targets from functional genomics screening approaches.

Emma J. Shanks1[*]

1Cancer Research UK: Beatson Institute, Garscube Estate, Switchback Road, Bearsden, Glasgow, G61 1BD, UK.

 

[*]Corresponding author: Emma Shanks PhD, Head of Screening, The Beatson Institute for Cancer Research, Garscube Estate, Switchback Road, Bearsden, Glasgow, G61 1BD, UK. Email: e.shanks@beatson.gla.ac.uk

Citation: Shanks EJ. Iterative data multiplexing (IDM) supports elucidation of drug targets from functional genomics screening approaches. Cancer Research Frontiers. 2015 Apr; 1(2): 119-126. doi: 10.17980/2015.119

Copyright: @ 2015 Shanks EJ. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Competing Interests: The author declares that there is no competing interests.

Received October 13, 2014; Revised February 13, 2015; Accepted March 5, 2015; Published March 27, 2015.

 

Abstract

The value of conducting high throughput functional genomic screening campaigns has been the subject of some debate over recent years, where a lack of reproducibility and preclusion of the identification of genuine genes of interest under a blanket of non-specific off-target effects have undermined confidence in the technology. High hopes for RNAi-based screening technologies to illuminate a suite of drug targets within different biological scenarios have arguably fallen short of our somewhat unrealistic expectations. But in the age of ‘Big Data’, where many of us are battling with large and complex datasets, it transpires that where RNAi screening may struggle to work independently, it thrives as a member of a larger team.

Key words: Iterative data multiplexing, big data, RNAi screening, High Content Analysis, target identification

 

 

 

 

 

 

 

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